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genequest module  (DNASTAR)


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    Structured Review

    DNASTAR genequest module
    Genequest Module, supplied by DNASTAR, used in various techniques. Bioz Stars score: 94/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genequest+module/GeneQuest/pmc12960631-138-16-20
    Average 94 stars, based on 113 article reviews
    genequest module - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    In Silico:

    Article Title: Genetic Diversity of Stellate Sturgeon in the Lower Danube River: The Impact of Habitat Contraction upon a Critically Endangered Population
    Article Snippet: Length quantification was performed by image analysis of the electrophoresis gel visualized in UV light, using a FastRuler Low Range DNA Ladder (Fermentas-SM1103). .. Additionally, the RFLP patterns from in silico digestions were inferred using the GeneQuest module from the Lasergene package [22]. ..

    Article Title: Characterization of phage AbpL with a terminally redundant genome and its therapeutic potential against drug-resistant Acinetobacter baumannii infections
    Article Snippet: .. The genomic sequence of phage AbpL was analyzed by in silico restriction enzyme digestion using the GeneQuest module of the DNASTAR Lasergene software ( https://www.dnastar.com/software/lasergene/ ). .. The genomic DNA of AbpL was digested with restriction endonucleases NdeI and HpaI (Thermo Fisher Scientific, USA), and the fragments were analyzed by agarose gel electrophoresis.

    Software:

    Article Title: Improved Prefusion Stability, Optimized Codon Usage, and Augmented Virion Packaging Enhance the Immunogenicity of Respiratory Syncytial Virus Fusion Protein in a Vectored-Vaccine Candidate
    Article Snippet: .. The codon usage of the different versions of RSV F was analyzed using the software Lasergene; the human codon usage data are from the Codon Usage Database ( http://www.kazusa.or.jp/codon/ ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 ORF b No. of nucleotides % GC content c No. of CpG dinucleotides d CpG dinucleotide frequency e (%) Non-HEK/non-opt 1,725 34.7 12 0.70 GA 1,725 57.6 100 5.80 GS 1,725 49.2 66 3.83 N 1,548 41.3 28 1.81 P 1,791 41.1 31 1.73 M 1,056 40.0 13 1.23 F 1,620 35.6 17 1.05 HN 1,719 36.1 16 0.93 L 6,702 33.4 68 1.01 Open in a separate window a Sequences were analyzed with the GeneQuest module in the Lasergene 13 software package (DNASTAR). b Coding sequences of ORFs of RSV F (non-HEK/non-opt, GA, and GS) and rB/HPIV3 structural proteins (N, P, M, F, HN, and L) as illustrated in were analyzed. c Percentage of G and C nucleotides in the ORF. d Number of CpG dinucleotides in the ORF. e Frequency of CpG dinucleotides in the ORF. ..

    Article Title: Characterization of phage AbpL with a terminally redundant genome and its therapeutic potential against drug-resistant Acinetobacter baumannii infections
    Article Snippet: .. The genomic sequence of phage AbpL was analyzed by in silico restriction enzyme digestion using the GeneQuest module of the DNASTAR Lasergene software ( https://www.dnastar.com/software/lasergene/ ). .. The genomic DNA of AbpL was digested with restriction endonucleases NdeI and HpaI (Thermo Fisher Scientific, USA), and the fragments were analyzed by agarose gel electrophoresis.

    Article Title: Expression of a novel mRNA transcript for human microsomal epoxide hydrolase (EPHX1) is regulated by short open reading frames within its 5?-untranslated region
    Article Snippet: Twenty-four hours after transfection, cells were harvested for luciferase reporter assay using the Dual-Glo Reporter Assay (Promega) and Veritas Microplate Luminometer (Turner Biosystems). .. The secondary structures of EPHX1 E1-b′ mRNA 5′ UTRs were modeled using the GeneQuest module of Lasergene version 6 software (DNASTAR). .. All statistical analyses were performed using GraphPad Prism v4.00 for Windows (GraphPad Software Inc.).

    Sequencing:

    Article Title: Regulation of the cd38 promoter in human airway smooth muscle cells by TNF-α and dexamethasone
    Article Snippet: .. The cd38 promoter sequence was examined for the presence of typical consensus elements using the GeneQuest module of Lasergene 6.0 program from DNASTAR. ..

    Article Title: Characterization of phage AbpL with a terminally redundant genome and its therapeutic potential against drug-resistant Acinetobacter baumannii infections
    Article Snippet: .. The genomic sequence of phage AbpL was analyzed by in silico restriction enzyme digestion using the GeneQuest module of the DNASTAR Lasergene software ( https://www.dnastar.com/software/lasergene/ ). .. The genomic DNA of AbpL was digested with restriction endonucleases NdeI and HpaI (Thermo Fisher Scientific, USA), and the fragments were analyzed by agarose gel electrophoresis.

    Article Title: Identification of cis -Regulatory Elements in the dmyc Gene of Drosophila Melanogaster
    Article Snippet: .. Most protein-coding genes of Drosophila contain a downstream core promoter element that functions cooperatively with an initiator to facilitate the binding of transcription factors in the absence of a TATA box., A search for consensus Drosophila Inr (T-C-A +1 -G/T-T-T/C) and downstream core promoter elements (G-A/T-C-G) using DNASTAR Lasergene 9.1, GeneQuest module, revealed the presence of downstream promoter sequence motifs comparable with the Drosophila consensus Inr/downstream core promoter element. .. Sequence motifs were typed in GeneQuest “type in pattern function” and searched for with a threshold of 100% (no errors allowed).

    Binding Assay:

    Article Title: Regulation of the cd38 promoter in human airway smooth muscle cells by TNF-α and dexamethasone
    Article Snippet: .. The GeneQuest module of Lasergene 6.0 program from DNASTAR was used to identify the potential transcription factor binding sites in the cd38 promoter. .. The 3 kb sequence of the cd38 promoter was analyzed using GeneQuest for the potential transcription factor binding sites using tfd.dat file.

    Article Title: Identification of cis -Regulatory Elements in the dmyc Gene of Drosophila Melanogaster
    Article Snippet: .. Most protein-coding genes of Drosophila contain a downstream core promoter element that functions cooperatively with an initiator to facilitate the binding of transcription factors in the absence of a TATA box., A search for consensus Drosophila Inr (T-C-A +1 -G/T-T-T/C) and downstream core promoter elements (G-A/T-C-G) using DNASTAR Lasergene 9.1, GeneQuest module, revealed the presence of downstream promoter sequence motifs comparable with the Drosophila consensus Inr/downstream core promoter element. .. Sequence motifs were typed in GeneQuest “type in pattern function” and searched for with a threshold of 100% (no errors allowed).



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